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Testosterone (T) ELISA Kit

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  • Cat.No.: AE64802GE

  • Reactivity: General

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48T 96T
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Product Details

Species Reactivity General
UniProt N/A
Abbreviation T
Alternative Names RP11-459F1.1; MGC104817; TFT; OTTHUMP00000017588|T brachyury homolog|T brachyury-like|transcription factor T
Range 0.2-80 ng/mL
Sensitivity 0.1 ng/mL
Sample Type Serum, Plasma, Other biological fluids
Detection Method Competitive
Analysis Method Quantitive
Assay Duration 1-4.5h
Sample Volume 1-200 μL
Detection Wavelengt 450 nm

Test principle

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Testosterone and Horseradish Peroxidase (HRP) conjugated Testosterone. The competitive inhibition reaction is launched between with HRP labeled Testosterone and unlabeled Testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Testosterone in the sample. The color development is stopped and the intensity of the color is measured.

 

Product Overview


 

Components


Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 12 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 12 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 10 mL

Stop Solution

1 x 6 mL

Adhesive Films

4

 

Specificity

This assay has high sensitivity and excellent specificity for detection of General T. No significant cross-reactivity or interference between General T and analogues was observed.
 

Recovery

Matrices listed below were spiked with certain level of recombinant General T and the recovery rates were calculated by comparing the measured value to the expected amount of General T in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

 

Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of General T and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type

1：2

1：4

1：8

1：16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

 

Stability


 

Sample collection and storage


 

Kits storage instructions


 

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