Product Details
| Species Reactivity |
Escherichia coli |
| UniProt |
P06996 |
| Host |
Rabbit |
| Synonyms |
OMPA; ompC; meoA; par; b2215; JW2203; OMPC_ECOLI; Outer membrane protein 1B; Porin OmpC. |
| Concentration |
1.5 mg/mL |
| Form |
Lyophilized or Liquid |
| Purity |
≥90% by SDS-PAGE |
| Molecular Mass |
35 kDa |
| Ig Type |
IgG |
| Purification |
Rabbit Polyclonal IgG, the antibody was affinity-purified from rabbit antiserum by protein G. |
| Label |
None |
| Immunogen |
Recombinant Escherichia coli Outer membrane protein C protein. AA range: 22-367. |
| Applications |
ELISA |
Product Overview
ompC polyclonal antibody was produced in the rabbit immunized by using the Recombinant Escherichia coli Outer membrane protein C protein (22-367AA) as the immunogen. The target protein ompC forms pores that allow passive diffusion of small molecules across the outer membrane. The primary outer membrane proteins implicated in decreased permeability in E. coli are the porins OmpC and OmpF. This Rabbit anti-Escherichia coli (strain K12) ompC Polyclonal antibody was tested in the ELISA. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the ompC proteins of Escherichia coli-origin and may be used to detect the endogenous levels of ompC protein.
Specifity
The antibody is a rabbit polyclonal antibody raised against OMPC. It has been selected for its ability to recognize OMPC in ELISA Kit.
Usage
ELISA=1:500-2000
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Stability
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
Storage
Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving.
**Avoid repeated freeze-thaw cycles.**
Stroage Buffer
0.01M PBS (pH 7.0-7.4) with 50% glycerol, 0.03% Proclin 300.
Precautions
This product is for research use only, not for diagnostic or therapeutic use.
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