Product Details
Species Reactivity |
Mouse |
UniProt |
Polyclonal antibody preparation |
Host |
Rabbit |
Synonyms |
ROBO2; KIAA1568; Roundabout homolog 2 |
Concentration |
1 mg/mL |
Form |
Lyophilized or Liquid |
Purity |
≥90% by SDS-PAGE |
Molecular Mass |
151 kDa |
Ig Type |
IgG |
Purification |
Rabbit Polyclonal IgG, affinity purified by Protein A. |
Label |
None |
Immunogen |
Synthesized peptide derived from the Internal region of human Robo2. at AA rangle: 237-286 |
Applications |
IHC; IF; ELISA |
Product Overview
ROBO2 belongs to the ROBO family, part of the immunoglobulin superfamily proteins that are highly conserved from fly to human. The encoded protein is a receptor for SLIT2, molecules known to function in axon guidance and cell migration. Defects in this gene are the cause of vesicoureteral reflux type 2. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. The deduced 1,380-amino acid protein shares 56% identity with ROBO1. RT-PCR ELISA detected highest expression of ROBO2 in adult and fetal brain, adult ovary, and in most individual brain regions examined. Intermediate expression was detected in fetal liver and in adult lung, kidney, spleen, testis, and spinal cord. Little to no expression was detected in adult pancreas, heart, liver, and skeletal muscle.
Specifity
AKAP 250 Polyclonal Antibody detects endogenous levels of AKAP 250 protein.
Usage
IHC 1:100-300; IF 1:200-1000; ELISA 1:20000; Not yet tested in other applications.
Stability
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
Storage
Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Stroage Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Precautions
This product is for research use only, not for diagnostic or therapeutic use.