Doxycycline (DOX) ELISA Kit

This assay is based on the competitive enzyme immunoassay for the detection of DOX in the sample. The coupling antigens are pre-coated on the micro-well stripes. The DOX in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-DOX antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the DOX in it. This value is compared to the standard curve and the DOX concentration is subsequently obtained.
 

 

Reagents	Quantity	Reagents	Quantity
Assay plate (96 Wells)	1	Instruction manual	1
Standards	6 x 1 mL	Redissolving Solution (concentrate 2 x)	2 x 20 mL
Antibody	1 x 6 mL	HRP-Conjugate	1 x 6 mL
Wash Buffer (concentrate 20 x)	1 x 20 mL	Stop Solution	1 x 6 mL
Substrate A	1 x 6 mL	Substrate B	1 x 6 mL
Adhesive Films	4

 

 

Sample Type	Number	Recovery range (%)	Average(%)
Serum	10	90-101	96
EDTA plasma	10	89-97	93
Heparin plasma	10	91-99	95

 

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

Sample Type	1：2	1：4	1：8	1：16
Serum	78-89%	81-99%	92-103%	95-105%
EDTA plasma	91-101%	90-98%	93-101%	91-98%
Heparin plasma	92-103%	93-102%	92-99%	91-101%

 

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
 

Store at 2-8°C. Please refer to Instruction Manual.
 

Store at 2-8°C. Please refer to Instruction Manual.