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Recombinant Human Enhanced green fluorescent protein (EGFP) Protein

  • Cat.No.: AP50007HU

  • Reactivity: Human

To Purchase AP50007HU

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Product Details

Species Reactivity Human
Source Prokaryotic expression
Host E. coli
Synonyms GFP; EGFP
Concentration 1 mg/mL
Form Lyophilized or Liquid
Purity > 95% as determined by SDS-PAGE
Residues C5MKY7
Tag N-terminal His-tag and Trx-ta
Purification Affinity purification
Molecular Weight(MW) 26 kDa
Applications Western blotting,ELISA;SDS-PAGE;Antigen;

Product Overview

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.
 

Usage

Reconstitute in PBS or others.
 

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 

Storage

Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
 

Stroage Buffer

Lyophilized from 50mM Tris-HCL,0.5mM EDTA,50mM NaCl,pH 7.4
 

Precautions

This product is for research use only, not for diagnostic or therapeutic use.