Rabbit anti-human Insulin-like growth factors 1 (IGF-1) PolyClonal antibody

Insulin-like Growth Factor I is a member of the insulin superfamily. It was originally discovered as a mediator of growth hormone actions on somatic cell growth, but has also been shown to be an important regulator of cell metabolism, differentiation and survival. IGF-I is synthesized as a preproprotein that is proteolytically cleaved to generate the mature protein linked by three disulfide bonds. Mature IGF-I is highly conserved among mammals, with 100% sequence identity between the Porcine, bovine, porcine, equine and canine proteins. IGF-I is synthesized in the liver and multiple other tissues. It is found in blood and other body fluids as a complex with specific high affinity IGF binding proteins (IGFBP-1to-6).
 

The antibody is a Rabbit polyclonal antibody raised against IGF-1. It has been selected for its ability to recognize IGF-1 in ELISA Kit and western blotting.
 

WB=1:100-500 ELISA=1:500-1000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
 

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
 

Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
 

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
 

This product is for research use only, not for diagnostic or therapeutic use.