×

Rabbit anti-human Alpha-fetoprotein (AFP) PolyClonal antibody

  • Cat.No.: PA82187HU

  • Reactivity: Human

To Purchase PA82187HU

Size:
100μg 500μg
Price: Inquire

Certificate



Product Details

Species Reactivity Human
UniProt Polyclonal antibody preparation
Host Rabbit
Synonyms A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
Concentration 1 mg/mL
Form Lyophilized or Liquid
Purity ≥90% by SDS-PAGE
Molecular Mass
Ig Type IgG
Purification Rabbit Polyclonal IgG, affinity purified by Protein A.
Label
Immunogen Recombinant human Alpha-fetoprotein (AFP)
Applications SDS-PAGE; Western blotting; ELISA

Product Overview

AFP is a glycoprotein with a molecular weight of between 65,000 and 70,000 daltons including 4% of carbohydrate . During fetal development, AFP maintains high levels in the serum and drops to very low levels throughout the remainder of life. AFP is elevated in the malignant diseases of hepatocellular, testicular nonseminomatous origin, and occasionally of other entodermal origin. AFP may be slightly elevated or persisted in the patients with large hepatic metastases or viral hepatitis. AFP measurement is widely accepted as tumor marker and for monitoring the therapeutic effectiveness of hepatocellular cancer and nonseminomatous testicular cancer .
 

Specifity

The antibody is a Rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in ELISA Kit and western blotting.
 

Usage

WB=1:100-500 ELISA=1:500-1000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
 

Stability

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
 

Storage

Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
 

Stroage Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
 

Precautions

This product is for research use only, not for diagnostic or therapeutic use.